pedf  (R&D Systems)

Journal: The British journal of dermatology

Article Title: Insights into male androgenetic alopecia using comparative transcriptome profiling: hypoxia-inducible factor-1 and Wnt/β-catenin signalling pathways.

doi: 10.1111/bjd.21783

Figure Lengend Snippet: Figure 3 Validation of the expression of differentially expressed genes involved in the hypoxia-inducible factor (HIF)-1 and Wnt signalling path- ways. (a) Haematoxylin and eosin staining showed that the hair follicles (HFs) were anagen HFs and the bulb diameter was decreased in vertex HFs compared with occipital HFs. (b) The expression of HIF-1 pathway-related genes (EGLN1, EGLN3 and HMOX1) and Wnt signalling pathway- related genes (PEDF/SERPINF1, SFRP2 and LGR5) was examined in vertex HFs compared with occipital HFs. The mRNA expression of EGLN1, EGLN3, PEDF/SERPINF1 and SFRP2 was significantly higher in the vertex HFs compared by paired-sample t-tests. (c) Immunofluorescence staining showed increased protein expression of EGLN1, EGLN3, PEDF or SFRP2 in vertex HFs compared by paired-sample t-tests. **P < 001, *P < 005.

Article Snippet: For recombinant protein stimulation experiments, HFs were incubated with 100 ng mL−1 recombinant pigment epithelium-derived factor (PEDF) protein (HY-P7054; MedChemExpress, Monmouth Junction, NJ, USA) or 1 μg mL 1 recombinant secreted Frizzled-related protein 2 (SFRP2) protein (6838-FR-025; R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Biomarker Discovery, Expressing, Staining

Journal: The British journal of dermatology

Article Title: Insights into male androgenetic alopecia using comparative transcriptome profiling: hypoxia-inducible factor-1 and Wnt/β-catenin signalling pathways.

doi: 10.1111/bjd.21783

Figure Lengend Snippet: Figure 5 Effects of PEDF/SERPINF1 and SFRP2 on dermal papilla cells (DPCs) and hair follicles (HFs). (a) The mRNA expression levels of PEDF/ SERPINF1 and SFRP2 were significantly downregulated after corresponding small interfering (si)RNA transfection in cultured DPCs. (b) xCELLigence system detection showed that siPEDF/SERPINF1 or siSFRP2 treatment significantly increased the proliferation of DPCs compared with the negative control (NC) group DPCs. (c) Recombinant protein PEDF or SFRP2 treatment decreased the proliferation of DPCs. (d) Immunofluorescence staining assay identified decreased protein levels of PEDF and SFRP2 after siPEDF or siSFRP2 treatment of HFs. (e) PEDF/SERPINF1 knockdown increased the length of hair shafts, whereas PEDF recombinant protein significantly inhibited HF growth compared with the NC after 2 days of culture. (f) Treatment with siSFRP2 or recombinant SFRP2 protein showed no significant effect on HF growth. (g) Macroscopic images of cultured hair follicles in different groups were obtained every other day. (h, i) Quantification of hair cycle stage from macroscopic images of cultured hair follicles on day 6. A greater percentage of anagen HFs and a lower percentage of catagen HFs remained in the PEDF/SERPINF1 siRNA-treated group, and PEDF recombinant protein facilitated catagen transition and shortened the anagen stage. By contrast, siSFRP2 and recombinant SFRP2 showed no significant effect on the HF hair cycle. Data are expressed as the mean (SD) of each group. P-values were calculated using unpaired-sample t- tests. **P < 001, *P < 005.

Article Snippet: For recombinant protein stimulation experiments, HFs were incubated with 100 ng mL−1 recombinant pigment epithelium-derived factor (PEDF) protein (HY-P7054; MedChemExpress, Monmouth Junction, NJ, USA) or 1 μg mL 1 recombinant secreted Frizzled-related protein 2 (SFRP2) protein (6838-FR-025; R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Expressing, Transfection, Cell Culture, Negative Control, Recombinant, Staining, Knockdown

Journal: Materials Today Bio

Article Title: Transplantation of human embryonic stem cell-derived retinal pigment epithelial cells via injectable microfluidic-templated microgels for retinal regeneration

doi: 10.1016/j.mtbio.2025.101880

Figure Lengend Snippet: Specific functional expression of hESC-RPE cells cultured on Fib@GHMS microgels. A: qRT–PCR comparing the expression profiles of key genes between hESC-RPE cells on Fib@GHMS and in TCP cultures (n = 3). The results are presented as 'fold expression' values to highlight the enhanced expression levels of certain genes in Fib@GHMS cultures compared with adherent cultures (dashed line). Gene expression was normalized to the endogenous reference gene ( GAPDH ). Wilcoxon paired signed-rank tests were used to compare ΔCt values for each gene in Fib@GHMS and adherent cultures. B, C: The levels of secreted PEDF and VEGF in conditioned culture media. The error bars represent standard deviations. D: Immunostaining of hESC-RPE cells on the surface of Fib@GHMS using antibodies against MITF, RPE65, CRALBP, Laminin, Best1, ZO-1, and LRAT. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.

Article Snippet: The concentrations of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) were measured using VEGF and PEDF ELISA kits (Bosterbio, USA) on days 1, 2, 3, and 4, following the manufacturer's instructions.

Techniques: Functional Assay, Expressing, Cell Culture, Quantitative RT-PCR, Gene Expression, Immunostaining

Journal: Journal of nanobiotechnology

Article Title: Enhanced therapeutic effect of PEDF-loaded mesenchymal stem cell-derived small extracellular vesicles against oxygen-induced retinopathy through increased stability and penetrability of PEDF.

doi: 10.1186/s12951-023-02066-z

Figure Lengend Snippet: Fig. 1 Characterisation of MSCs and sEVs. A Representative images of adipogenic (oil red O staining), osteogenic (alizarin red S staining), and chondrogenic (alcian blue staining) differentiation assay. B Analysis of sizes of sEVs from each group using Nanosight. C Representative transmission electron micrograph for each group; scale bar = 200 nm. D Representative western blots for loaded proteins and markers (PEDF, CD63, TSG101, and CD9) in MSC-sEVs. E Flow cytometry for measuring the loading efficiency of PEDF in PEDF-sEVs. FITC-labelled PEDF was used. F The concentrations of PEDF in sEVs and PEDF-sEVs were determined using ELISA (n = 3/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: PEDF (1177- SF-025, R&D SYSTEMS, USA) was mixed with sEVs at a 1:5 concentration ratio.

Techniques: Staining, Differentiation Assay, Transmission Assay, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Journal of nanobiotechnology

Article Title: Enhanced therapeutic effect of PEDF-loaded mesenchymal stem cell-derived small extracellular vesicles against oxygen-induced retinopathy through increased stability and penetrability of PEDF.

doi: 10.1186/s12951-023-02066-z

Figure Lengend Snippet: Fig. 3 PEDF-sEVs suppress the expression of inflammatory cytokines in HRECs. A Starved HRECs were pre-treated with PEDF, sEVs, or PEDF-sEVs under stimulation with 10 ng/mL VEGF for 24 h. Representative western blot images showing the expression of ICAM-1 in HRECs. B Relative expression of ICAM-1 compared with that of GAPDH (n = 3). C, D, E. PCR analysis of the expression of TNF-α, VCAM-1, and IL-1β in HRECs (n = 5). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: PEDF (1177- SF-025, R&D SYSTEMS, USA) was mixed with sEVs at a 1:5 concentration ratio.

Techniques: Expressing, Western Blot